Many experimenters do not know much about the cleaning and disinfection of pipettes, which may bring external pollution to the experiment or affect the service life of pipettes and reduce its performance. Let's take a look at the common cleaning and disinfection methods of pipettes.
01 Daily cleaning, you only need to wipe the handle of the pipette, the ejector and the outside of the white sleeve repeatedly with 75% alcohol clean wipes to reduce the probability of contamination.
When the nozzle connecting piece enters the aqueous solution, buffer, acid or alkali solution due to back suction or placing the pipette horizontally, the lower part of the pipette needs to be disassembled, and the contaminated tube should be rinsed with pure water. Air dry in a ventilated place or put it in an oven (below 60℃) to dry and cool naturally, and then coat the piston with lubricating oil and then assemble the pipette.
02 When the organic solvent contaminates the pipette, it is different from the inorganic solution in that it is necessary to rinse the lower half of the pipette with neutral detergent or disassemble it directly to let the organic solvent volatilize and rinse with pure water, and dry it according to the above method , Apply lubricant to the piston.
03 When conducting cell experiments or experiments with potentially infectious liquids (it is recommended to use a pipette with a filter element or a pipette with a filter element to reduce pollution), the disinfection and cleaning of the pipette is more important, and you can Using ultraviolet radiation to kill surface microorganisms, this method will not affect the degree and accuracy of the pipette.
For pipettes that can be sterilized by high temperature and high pressure, they can be wrapped in sterile bags or kraft paper. Sterilize them for 20 minutes at 120°C and 1 bar atmospheric pressure. After sterilization is complete, dry them at room temperature and give the piston Apply oil before assembly. Whether it needs to be disassembled should be operated according to the requirements of different brands of pipettes.
For the daily maintenance of pipettes, the corresponding operations must be performed according to the instructions of each manufacturer, and there will be slight differences between different brands.