The 96-well plate of the microplate reader can be used to read the results of the enzyme-linked immunoassay kit, so the use of the kit must be standardized. The micro editor briefly describes how many hospitals use microplate readers to run at random rates. If the operating habits cannot be corrected in time, it will lead to greater errors.
It can be said that the microplate reader is a phase change photoelectric colorimeter or spectrophotometer. The working principle and main structure used are basically the same as the photoelectric colorimeter. The light wave emitted by the light source lamp is converted into monochromatic light through the filter, and enters the sample to be tested. Part of the monochromatic light is absorbed by the sample, and the other part is irradiated on the photodetector and converted into a corresponding electrical signal. Data processing and calculation are performed through pre-amplification, logarithmic amplification, etc., and the result is displayed. According to the experimental requirements, the microplate reader can be divided into movable and non-removable. There are 96-well plates and 384-well plates. What should I pay attention to when handling 96-well plates?
Pay attention to the following items in the operation of the 96-well plate of the microplate reader:
1. When using a liquid dispenser to add liquid, the liquid adding head cannot mix.
2. Clean the circuit board and clean it. If possible, use a washing machine to wash dishes to avoid cross contamination.
3. According to the instructions of the kit, the reaction time should be accurate.
4. Do not touch the microplate during measurement. Take care to prevent the microplate from squeezing your hands during transportation.
5. Do not spill samples or reagents on the surface or inside of the instrument. Wash hands after surgery.
6. If the samples or reagents used are harmful to pollution, toxicity and biological characteristics, the operation instructions of the kit must be strictly followed to avoid harm to the operators. After the instrument is exposed to contaminants or infectious substances, it must be cleaned and disinfected.
7. Do not turn off the power during measurement.
8. For the deviation of the measurement results caused by the kit, the parameters should be modified according to the actual situation to achieve better results.
After using the instrument, please put on the dust cover. Be careful not to disassemble the microplate reader.