Regarding the microplate reader, we need to know that it is a phase change photoelectric colorimeter or a spectrophotometer. The working principle and main structure used are basically the same as the photoelectric colorimeter. The light wave emitted by the light source lamp is converted into monochromatic light by the filter and enters the sample to be tested. Next, the editor will introduce to you the functions of the 96-well plate and the precautions for operation!
Part of the monochromatic light is absorbed by the sample, and the other part is irradiated on the photodetector to be converted into a corresponding electrical signal, and data processing and calculation are performed through pre-amplification, logarithmic amplification, etc., and the results are displayed. According to the experimental requirements, the microplate reader can be divided into detachable and non-detachable. There are 96 wells and 384 wells.
So, how should we correctly operate the 96-well plate, and what should we pay attention to when operating?
The 96-well function of the microplate reader is used to read the results of the enzyme-linked immunoassay kit, so the use of the kit must be standardized. Many hospitals use microplate readers to run at random rates. If the operating habits cannot be corrected in time, it will lead to greater errors.
Therefore, the following matters should be paid attention to in the operation of the 96-well plate of the microplate reader:
1. When using the liquid dispenser to add liquid, the liquid adding head cannot mix.
2. Clean the circuit board and clean it. If possible, use a washing machine to clean the plates to avoid cross-contamination.
3. According to the instructions of the kit, the reaction time should be accurate.
4. Do not touch the microplate during measurement. Take care to prevent the microplate from squeezing your hands during transportation.
5. Do not spill samples or reagents on the surface or inside of the instrument. After the operation is complete, be sure to wash your hands.
6. If the samples or reagents used are harmful to pollution, toxicity and biological characteristics, the operation instructions of the kit must be strictly followed to avoid harm to the operators. After contact with contaminated or infectious materials, the instrument must be cleaned and disinfected.
7. Do not turn off the power during measurement.
8. For the deviation of the measurement results caused by the kit, the parameters should be modified according to the actual situation to achieve better results.