96-well plates are a very useful device for cell culture. This device is very common in certain areas. Today we will introduce you to 96-well boards. Which methods, let Xiaobian introduce you in detail below.
After taking the board, pick up the 96-well plate with your hand, shake it several times, then turn it back to a few times. However, in this method, the cells in the five porous 2-3 holes are always unevenly distributed, and in most cases, the cells are distributed in the middle of the holes, which makes me worry for a while. . I also saw that another person put the planting plate on the shaker and shaken a few minutes. The result is worse. All cells ran to the middle.
Typically, a 100 microliter cell suspension is added to each of the 96-well plates, and is added from the left side of the hole near the bottom. After adding half of the plate, mix the cell suspension, do not join again, continue to add the remaining half plate, add the tablet on the back, gently hold the left side of the tablet with the left hand, and tap the right edge of the tablet with the right hand, and pay attention to master Strength (I usually tap three times). If it is too strong or too many, the cells will be concentrated and accumulated. Clockwise rotate the tablet (the counterclockwise effect is not good), sequentially tap the remaining three sides, stand for about 5 minutes, put in the 37 degree culture box.
New method: This method is great. It is evenly planted. Use a pipette to absorb evenly blown cells, then press the gun on a side wall of the hole (pay attention to the bottom of the gun tip), then slowly tap the cells in the gun, do not shake after planting The board is placed directly under the microscope, which can be said to be 100% effective. I hope this is helpful for everyone.