What problems should we pay attention to when using 96-well plates!
In biological laboratories, 96-well plates, also known as 96-well cell culture plates. It is made of imported optically clear pure polystyrene. It is made by a special process to achieve better adsorption of cells, and all products are sterilized by gamma rays. In order to let everyone have a deeper understanding of the 96-well plate, I will take you to think about three questions.
1. How to maintain the uniformity of the 96-well plate
There are two types of floors, one that is uniform between the holes and the other that is uniform within the holes.
To achieve uniformity between wells, it is recommended that the landlord use a multi-channel pipette and use the corresponding sample jar. Adding each well one by one makes it difficult to achieve uniformity.
I have no secrets about the uniformity of the holes. After adding the cell suspension, the experience is not to shake or tap the plate, but directly into the incubator to make it stick to the wall.
2. Precautions for the use of 96-well plate
As with normal cell culture, using 96-well plates. Be careful with your cells, media, and utensils. Pay particular attention to the direction of the airflow during operation. Do not move germs or objects with your hands.
3. What is the principle of antigen-binding on a 96-well plate when performing ELISA?
Coating antigens used in ELISAs are usually proteins, and the forces that bind proteins to the plate matrix are usually hydrophobic. Different proteins have different binding constants due to their different hydrophobicities. Since generally proteins have hydrophilic groups on their periphery and hydrophobic groups are encapsulated inside, proteins can be "processed" to increase their binding.
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