In biological experiments, experimental operators are generally drawn by artificial or semi-automatic rolls, and when encountering large-size orifice coils, it is easy to happen or leak samples, affecting the accuracy of the experiment. Even the waste of interruption or failure, time, reagents, supplies, etc. of the entire experiment. The 384-well plate has a large number of holes and intensive, and the experimental operators must be highly concentrated, and their own emotions or slight interferences of the outside world will inevitably create distinctive and memory failures, and the plurality of plus errors occur. So how do you use 384-well plates correctly? The following small series is introduced to you.
After adding the internal standard, it can be partially eliminated inaccurate the quantity of the final product, but even if so, the conventional quantification method can only be counted as a half-quantitative, coarse quantitative method. The fluid flowing in the horizontal pipe has dynamic pressure and static pressure, under certain conditions, these two forms of energy can be converted to each other, but the sum of energy is constant. During the design of the throttle device, the outer diameter of the 384-well plate has a gap at the outer diameter of the ring chamber, and it is assumed that the hole plate and the ring chamber temperature are the same, but in fact, it is not possible, the orifice plate is surrounded by the ring chamber, and there is The high temperature fluid provides heat, so the temperature is high, which is fully expanded. After finding the root cause of the deformation of the orifice, the prevention of the deformation is simple and effective, that is, when designing the calculation, considering the temperature of the ring chamber may be much lower than the actual situation of the hole plate, a reasonable calculation gap. There is often a modification in the working conditions, which will cause the instrument to be accurately measured. The deformation of the orifice is often a throttle of a large-hole plate flow meter, a temperature having a high temperature of the medium, such as an overheating steam, and a 384 well plate thickness relatively thin ring chamber.
The effect of temperature on the flow meter and its correction, the fluid temperature change causes a change in density, resulting in a change in relationship between differential pressure and flow, secondly, temperature change causes a variation of the inner diameter of the pipe, 384 orifice opening, and changes in temperature Correct, it is to take a temperature meter to measure the on-site temperature in the secondary meter to correct the error. When the steam is measured, the inflatable correction of the fluid must be performed, and the specific correction coefficient can be referred to the throttling device design manual. The flow coefficient of the convexual flow meter and the Renault number have a certain relationship. When the mass flow changes, the Renault count is proportional to the change, thus causing the flow rate to change. Of course, the balancing valve and pressure valve can be turned off, and open a valve to introduce high temperature steam to cool into cold water. This method is due to too high of the steam temperature, which is easy to damage the instrument, generally not used. Especially pay attention to measuring high temperature steam, the conductive tube is not fully filled with water or the temperature is strictly prohibited to invest in. The compressed air is measured, and the cold water directly uses the measured medium conductors.